year 18, Issue 72 (11-2019)
J. Med. Plants 2019, 18(72): 183-191 |
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Yadegari M, Mirdar S, Hamidian G, Berenjeian Tabrizi H. Effect of the Adiantum capillus-veneris Extract on Immunohistochemical Expression of Lung Heat-Shock Proteins in Rats Exposed to Hypoxia. J. Med. Plants 2019; 18 (72) :183-191
URL: http://jmp.ir/article-1-2713-en.html
URL: http://jmp.ir/article-1-2713-en.html
1- PhD of Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Mazandaran, Iran
2- Professor, PhD of Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences,University of Mazandaran, Mazandaran, Iran
3- Assistant Professor, PhD of Histological, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
4- Assistant Professor, Department of Physical Education and Sport Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran , hberenjeian@gmail.com
2- Professor, PhD of Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences,University of Mazandaran, Mazandaran, Iran
3- Assistant Professor, PhD of Histological, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
4- Assistant Professor, Department of Physical Education and Sport Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran , hberenjeian@gmail.com
Abstract: (3363 Views)
Background: Despite the traditional use of the Adiantum capillus-veneris plant, there is little evidence of its clinical effects.
Objective: The purpose of the present study was to investigate the effect of the Adiantum capillus-veneris extract on Immunohistochemical expression of Lung heat-Shock Proteins in rats exposed to hypoxia.
Methods: Twenty-four male Wistar rats (control: 8, training-hypoxia: 8, training-hypoxia-supplement: 8) without clinically evident disease were used. The rats were exposed to hypoxia environment for 3 week following 6 weeks interval training. Tweleve of the experimental samples were taken 500 ml Adiantum capillus-veneris extract per body weight (kg) in during exposure to hypoxia environment. Finally lung tissue was removed for immunohistochemistry tests of HSP70 and HSP90. To analyze of data, ANOVA test was used (α≤0.05).
Results: Expression of HSP70 and HSP90 increased significantly in hypoxia group comparison with the control group (P≤0.05). Expression of HSP70 and HSP90 protein decreased significantly in the Supplement hypoxia group comparison with the hypoxia group (P≤0.05).
Conclusion: Reduction effects of Adiantum capillus-veneris extract on expression of the parenchyma lung heat- shock protein in hypoxia conditions was observed that probably indicate decreased oxidative stress in the lung.
Objective: The purpose of the present study was to investigate the effect of the Adiantum capillus-veneris extract on Immunohistochemical expression of Lung heat-Shock Proteins in rats exposed to hypoxia.
Methods: Twenty-four male Wistar rats (control: 8, training-hypoxia: 8, training-hypoxia-supplement: 8) without clinically evident disease were used. The rats were exposed to hypoxia environment for 3 week following 6 weeks interval training. Tweleve of the experimental samples were taken 500 ml Adiantum capillus-veneris extract per body weight (kg) in during exposure to hypoxia environment. Finally lung tissue was removed for immunohistochemistry tests of HSP70 and HSP90. To analyze of data, ANOVA test was used (α≤0.05).
Results: Expression of HSP70 and HSP90 increased significantly in hypoxia group comparison with the control group (P≤0.05). Expression of HSP70 and HSP90 protein decreased significantly in the Supplement hypoxia group comparison with the hypoxia group (P≤0.05).
Conclusion: Reduction effects of Adiantum capillus-veneris extract on expression of the parenchyma lung heat- shock protein in hypoxia conditions was observed that probably indicate decreased oxidative stress in the lung.
Type of Study: Research |
Subject:
Pharmacology & Toxicology
Received: 2018/07/13 | Accepted: 2018/09/2 | Published: 2019/10/28
Received: 2018/07/13 | Accepted: 2018/09/2 | Published: 2019/10/28
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